Ozone disinfection, or ozonation, Ozone is an unstable molecule which readily gives up one atom of oxygen providing a powerful oxidizing agent which is toxic to most waterborne organisms. It is a very strong, broad spectrum disinfectant that is widely used in Europe and in a few municipalities in the United States and Canada. It is an effective method to inactivate harmful protozoa that form cysts. It also works well against almost all other pathogens. Ozone is made by passing oxygen through ultraviolet light or a "cold" electrical discharge. To use ozone as a disinfectant, it must be created on-site and added to the water by bubble contact. Some of the advantages of ozone include the production of fewer dangerous by-products and the absence of taste and odour problems (in comparison to chlorination). No residual ozone is left in the water.[13] In the absence of a residual disinfectant in the water, chlorine or chloramine may be added throughout a distribution system to remove any potential pathogens in the distribution piping.
The Lifestraw go simplifies water purification by allowing users to scoop water from a river or other unsafe water source into the bottle, screw the lid on, and sip clean water through the mouthpiece. We have not had the opportunity to test the Lifestraw go. We would be interested in comparing it to the Sawyer Personal Water Bottle. Our next post will be a test of the Sawyer bottle.

In some systems, the carbon prefilter is omitted, and a cellulose triacetate membrane is used. CTA (cellulose triacetate) is a paper by-product membrane bonded to a synthetic layer and is made to allow contact with chlorine in the water. These require a small amount of chlorine in the water source to prevent bacteria from forming on it. The typical rejection rate for CTA membranes is 85–95%.

Many books and articles suggest this method as a safe alternative when lacking water filtration or purification methods. Without testing equipment some methods are difficult to prove. Norseman of Survivology 101 posted two great blogs which include testing done while he trained with the Norwegian school of Winter Warfare. The testing shows that the Mash or Seep showed zero improvement in lowering the bacterial count. Norseman is a retired Marine who held a Scout Sniper Survival instructor position at the First Marine Division, and SERE instructor.
The first part of the purification tag team must eliminate microorganisms, like harmful bacteria and parasites. There are a handful of tried and true methods for doing this. The most familiar is boiling. Simply bringing water up to its boiling point of 212 degrees Fahrenheit will kill almost all microorganisms, so just a few minutes of boiling will do the job.